Pasteurization, storage conditions, and virus concentration methods influence RT-qPCR detection of SARS-CoV-2 RNA in wastewater


This article was originally published here

Sci Total Environ. 2022 Jan 25:153228. doi: 10.1016/j.scitotenv.2022.153228. Online ahead of print.

ABSTRACT

The COVID-19 pandemic presents many public health challenges, including tracking infected people from local to regional scales. Viral RNA wastewater monitoring has emerged as a complementary approach to track and monitor the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus in various land use and size communities. of different populations. In the present study, we investigate how five different parameters (pasteurization, storage temperature, storage time, polyethylene glycol (PEG) concentration, and pellet mass) affect the detection of the SARS-CoV-2 N gene and indicator faecal abundance mild pepper mottle virus gene (PMMoV). Pretreatment of composite 24-hour wastewater samples (n = 14) by pasteurization at 60°C resulted in a significant reduction in total RNA concentration and SARS-CoV-2 N/L gene copies ( paired Student’s t test, P

PMID:35090920 | DO I:10.1016/j.scitotenv.2022.153228

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