Determination of the optimal concentration of vitamin E in polyethylene inserts to produce a minimal biological response to prosthetic wear debris


This article was originally published here

J Biomed Mater Res B Appl Biomater. 2022 Feb 5. doi:10.1002/jbm.b.35019. Online ahead of print.

ABSTRACT

The introduction of Ultra High Molecular Weight Polyethylene blended with Vitamin E (VE-UHMWPE) for use in prosthetic components of hip implants has resulted in the production of implants that have excellent mechanical properties and significantly less adverse cellular responses. Considering the importance of a biological response to wear in the survival of a prosthesis, we generated wear debris from UHMWPE that had been prepared with different concentrations of vitamin E from 0.1 , 0.3, 0.5 and 1% and evaluated their biological reaction in vitro and in vivo. All types of VE-UHMWPE debris promoted significantly lower expression of Tnf-α in murine peritoneal macrophages than that induced by conventional UHMWPE debris. However, Tnf-α levels were not significantly different among macrophages that were stimulated with VE-UHMWPE wear at the concentrations tested. The ability of wear debris to induce inflammatory osteolysis was evaluated in a model of cranial osteolysis in mice. Expressions of Tnf-α, Il-6 and Rankl in granulomatous tissue formed around wear debris were significantly reduced in mice that were implanted with 0.3% VE-UHMWPE debris compared to corresponding values ​​for mice that had been implanted with UHMWPE Debris. Consistent with this finding, 0.3% VE-UHMWPE debris showed the lowest osteolytic activity as evidenced by reduced bone resorption area, degree of inflammatory cell infiltration, and TRAP staining area. Our results suggest that a vitamin E concentration of 0.3% is the most appropriate concentration for use in prosthetic components with reduced adverse cellular response to prolong implant life.

PMID:35122380 | DO I:10.1002/jbm.b.35019

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